Site Directed Recombination

Site directed recombination consists in forcing recombination to occur at a defined location. Classical homologous gene targeting cannot be considered as site directed recombination for it implies only the selection of recombination at the desired locus among what is often a sea of non-targeted events.

In contrast, any process that would channel most recombination events in the targeted locus would be site directed recombination. Cellectis’ MRS© are ideal tools for site directed recombination: delivering a double-strand break in the locus targets the vast majority of recombination event to this locus, by homologous recombination.