InVoLin®

InVoLin® stands for in vivo linearization. It refers to a specific MRS©, where the transgene is excised from a circular plasmid within the cell. This MRS© does not rely on homologous recombination. It is a tool for improving the efficiency of classical transgenesis.

Classical transgenesis relies on the transfection of foreign sequences into the nucleus The best efficiencies are usually obtained after in vitro linearization of the transgene. It is excised from its plasmid vector, and the purified DNA fragment is introduced into the cell. However, even in these conditions, many problems are encountered, including:

• Efficiency, which can be very low, depending on the organism.
• Number of copies, which can be very high, often resulting in silencing.
• Integrity of the copies.

InVoLin® improves these three parameters, making transgenesis accessible to a number of resistant cell and species. Impressive results were obtained in fish (see the Publication web page).